Home > Sandwich ELISA Kits > Human Surfactant Associated Protein D (SP-D) ELISA Kit

Product Name:

Human Surfactant Associated Protein D (SP-D) ELISA Kit

Cat.#:

JL46777

Regulatory Status:

RUO

Alternative:

SFTPD; COLEC7; PSP-D; SFTP4; SP-D; Pulmonary Surfactant Protein D; Collectin-7; Lung surfactant protein D

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

1.56-100ng/mL

Sensitivity:

0.75ng/mL

Specificity:

This kit recognizes Human SPD in samples. No significant cross-reactivity or interference between Human SPD and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Immunology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| SPD

Surfactant Associated Protein D (SPD) is a pattern-recognition molecule that is an important component of the mucosal immune system of the reproductive tract (FRT). In addition to its host defense function in the FRT, it has an immunomodulatory role in labor and preterm delivery. Its deficiency affects the fertility of female organisms, highlighting the role of SPD in ovarian and uterine physiology.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	100	50	25	12.5	6.25	3.12	1.56	0
OD	2.58	1.59	0.93	0.55	0.37	0.28	0.21	0.09
Corrected OD	2.49	1.5	0.84	0.46	0.28	0.19	0.12	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human SPD spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	83-101	95
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human SPD and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	86-108	105-115
1 : 4	Average (%)	94	97	108